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mouse polyclonal nucleolin antibody (Danaher Inc)

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Danaher Inc mouse polyclonal nucleolin antibody

(A) Schematic shows the experimental design for the assessment of a human melanoma xenograft lung metastasis model following treatment with Np75-4A22. (B) Live-monitoring of lung metastatic tumor growth of A875-NT melanoma cells using the Xenogen IVIS-200 luciferase imaging system. Bioluminescent imaging of luciferase expressing A875-NT melanoma cells (control) in the lungs of SCID mice was measured 1, 2 and 3-weeks following treatment with Np75-4A22 (200 mg/kg/day) or vehicle alone. Representative images taken 3 weeks following implantation are shown (left panel). Graph shows the average quantification of luciferase signal (N=8-10 mice/group; right panel). Shown is one representative experiment from N=3 independent experiments. Data are expressed as the mean ± SEM. Means that are significantly different between treatments as analyzed by Student t-test are indicated. *, p<0.05: **, p<0.01. (C) Histological analysis of lung tumor burden in SCID mice bearing A875-NT or A875-shp75 tumors 3 weeks following of treatment with Np75-4A22 or vehicle alone. Left panel shows representative images of formalin fixed paraffin embedded lung sections stained by immunohistochemistry with a human-specific <t>nucleolin</t> antibody (brown) to detect the presence of tumor cells. Sections were counterstained with hematoxylin (blue). Scale bar, 60μm. Right panel shows quantification of the number of cancer cells per lung tissue section. Five non-sequential lung sections, 100 μm apart, were counted and averaged for each mouse (N=8-10 mice per group). Data is expressed as the mean ± SEM. Means that are significantly different are indicated. A875-NT vehicle vs Np75-4A22 ** p=0.0179; A875-NT vs A875-shp75 # p=0.0367; A857-shp75 vehicle vs Np75-4A22 not significant (n.s.), one-way ANOVA with Tukey’s multiple comparisons test.

Mouse Polyclonal Nucleolin Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+polyclonal+nucleolin+antibody/bio_rxiv__2023__11__13__566904-169-1-7
Average 86 stars, based on 1 article reviews

mouse polyclonal nucleolin antibody - by Bioz Stars, 2026-09

86/100 stars

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1) Product Images from "Impaired migration and metastatic spread of human melanoma by a novel small molecule targeting the transmembrane domain of death receptor p75 NTR"

Article Title: Impaired migration and metastatic spread of human melanoma by a novel small molecule targeting the transmembrane domain of death receptor p75 NTR

Journal: bioRxiv

doi: 10.1101/2023.11.13.566904

Figure Legend Snippet: (A) Schematic shows the experimental design for the assessment of a human melanoma xenograft lung metastasis model following treatment with Np75-4A22. (B) Live-monitoring of lung metastatic tumor growth of A875-NT melanoma cells using the Xenogen IVIS-200 luciferase imaging system. Bioluminescent imaging of luciferase expressing A875-NT melanoma cells (control) in the lungs of SCID mice was measured 1, 2 and 3-weeks following treatment with Np75-4A22 (200 mg/kg/day) or vehicle alone. Representative images taken 3 weeks following implantation are shown (left panel). Graph shows the average quantification of luciferase signal (N=8-10 mice/group; right panel). Shown is one representative experiment from N=3 independent experiments. Data are expressed as the mean ± SEM. Means that are significantly different between treatments as analyzed by Student t-test are indicated. *, p<0.05: **, p<0.01. (C) Histological analysis of lung tumor burden in SCID mice bearing A875-NT or A875-shp75 tumors 3 weeks following of treatment with Np75-4A22 or vehicle alone. Left panel shows representative images of formalin fixed paraffin embedded lung sections stained by immunohistochemistry with a human-specific nucleolin antibody (brown) to detect the presence of tumor cells. Sections were counterstained with hematoxylin (blue). Scale bar, 60μm. Right panel shows quantification of the number of cancer cells per lung tissue section. Five non-sequential lung sections, 100 μm apart, were counted and averaged for each mouse (N=8-10 mice per group). Data is expressed as the mean ± SEM. Means that are significantly different are indicated. A875-NT vehicle vs Np75-4A22 ** p=0.0179; A875-NT vs A875-shp75 # p=0.0367; A857-shp75 vehicle vs Np75-4A22 not significant (n.s.), one-way ANOVA with Tukey’s multiple comparisons test.

Techniques Used: Luciferase, Imaging, Expressing, Formalin-fixed Paraffin-Embedded, Staining, Immunohistochemistry

p75 NTR immunostaining (red) in lung metastasis induced by A875-NT (A) or shp75-A875 (B) cells counter-stained for human nucleolin (green) and DAPI (white). Scale bar, 100μM.

Figure Legend Snippet: p75 NTR immunostaining (red) in lung metastasis induced by A875-NT (A) or shp75-A875 (B) cells counter-stained for human nucleolin (green) and DAPI (white). Scale bar, 100μM.

Techniques Used: Immunostaining, Staining

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Journal: bioRxiv

Article Title: Impaired migration and metastatic spread of human melanoma by a novel small molecule targeting the transmembrane domain of death receptor p75 NTR

doi: 10.1101/2023.11.13.566904

Figure Lengend Snippet: (A) Schematic shows the experimental design for the assessment of a human melanoma xenograft lung metastasis model following treatment with Np75-4A22. (B) Live-monitoring of lung metastatic tumor growth of A875-NT melanoma cells using the Xenogen IVIS-200 luciferase imaging system. Bioluminescent imaging of luciferase expressing A875-NT melanoma cells (control) in the lungs of SCID mice was measured 1, 2 and 3-weeks following treatment with Np75-4A22 (200 mg/kg/day) or vehicle alone. Representative images taken 3 weeks following implantation are shown (left panel). Graph shows the average quantification of luciferase signal (N=8-10 mice/group; right panel). Shown is one representative experiment from N=3 independent experiments. Data are expressed as the mean ± SEM. Means that are significantly different between treatments as analyzed by Student t-test are indicated. *, p<0.05: **, p<0.01. (C) Histological analysis of lung tumor burden in SCID mice bearing A875-NT or A875-shp75 tumors 3 weeks following of treatment with Np75-4A22 or vehicle alone. Left panel shows representative images of formalin fixed paraffin embedded lung sections stained by immunohistochemistry with a human-specific nucleolin antibody (brown) to detect the presence of tumor cells. Sections were counterstained with hematoxylin (blue). Scale bar, 60μm. Right panel shows quantification of the number of cancer cells per lung tissue section. Five non-sequential lung sections, 100 μm apart, were counted and averaged for each mouse (N=8-10 mice per group). Data is expressed as the mean ± SEM. Means that are significantly different are indicated. A875-NT vehicle vs Np75-4A22 ** p=0.0179; A875-NT vs A875-shp75 # p=0.0367; A857-shp75 vehicle vs Np75-4A22 not significant (n.s.), one-way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: Human-specific mouse polyclonal nucleolin antibody was from Abcam (#ab136649).

Techniques: Luciferase, Imaging, Expressing, Formalin-fixed Paraffin-Embedded, Staining, Immunohistochemistry

Journal: bioRxiv

Article Title: Impaired migration and metastatic spread of human melanoma by a novel small molecule targeting the transmembrane domain of death receptor p75 NTR

doi: 10.1101/2023.11.13.566904

Figure Lengend Snippet: p75 NTR immunostaining (red) in lung metastasis induced by A875-NT (A) or shp75-A875 (B) cells counter-stained for human nucleolin (green) and DAPI (white). Scale bar, 100μM.

Article Snippet: Human-specific mouse polyclonal nucleolin antibody was from Abcam (#ab136649).

Techniques: Immunostaining, Staining

Fig. 2 Expression analysis of Mina53 and Ki-67 in cultured cells and surgically resected formalin- fixed ESCC tissues. A, subcellular localization of Mina53. The localization of Mina53 in TE-11 was visualized by indirect immunofluorescence stain- ing with anti-Mina53 antibody (a). Cells were also stained with antibody against nucleolin (b). An overlapped image is also shown (c). B, H&E stain- ing of a section containing a well-differentiated ESCC. C, control section for B in which the pri- mary antibody was omitted. D, a serial section of B stained by anti-Mina53 antibody showing ele- vated expression of Mina53 in the tumor area and little staining in the adjacent nonneoplastic tissue. E, enlargement of the section shown in D showing characteristic nuclear localization of Mina53. F, a serial section of B stained by anti–Ki-67 antibody. Positive staining is brown, and counterstained nu- clei are blue (CF). Scale bars: 100 m in BD and F; 50 m in E.

Journal: Clinical Cancer Research

Article Title: Mina53 as a Potential Prognostic Factor for Esophageal Squamous Cell Carcinoma

doi: 10.1158/1078-0432.ccr-03-0543

Figure Lengend Snippet: Fig. 2 Expression analysis of Mina53 and Ki-67 in cultured cells and surgically resected formalin- fixed ESCC tissues. A, subcellular localization of Mina53. The localization of Mina53 in TE-11 was visualized by indirect immunofluorescence stain- ing with anti-Mina53 antibody (a). Cells were also stained with antibody against nucleolin (b). An overlapped image is also shown (c). B, H&E stain- ing of a section containing a well-differentiated ESCC. C, control section for B in which the pri- mary antibody was omitted. D, a serial section of B stained by anti-Mina53 antibody showing ele- vated expression of Mina53 in the tumor area and little staining in the adjacent nonneoplastic tissue. E, enlargement of the section shown in D showing characteristic nuclear localization of Mina53. F, a serial section of B stained by anti–Ki-67 antibody. Positive staining is brown, and counterstained nu- clei are blue (CF). Scale bars: 100 m in BD and F; 50 m in E.

Article Snippet: Rabbit anti–c-Myc polyclonal antibody (sc-764; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-nucleolin polyclonal antibody (sc-13057; Santa Cruz Biotechnology), Alexa 488-conjugated goat antimouse IgG (Molecular Probes, Eugene, OR), goat anti-mouse IgG-horseradish peroxidase and Cy3-conjugated anti-rabbit IgG (Zymed Laboratories, South San Francisco, CA), anti– -actin monoclonal antibody (AC-15; Sigma-Aldrich Japan, Tokyo, Japan), mouse anti–Ki-67 antibody (clone MIB-1; DAKO A/S, Glostrup, Denmark), biotinylated rabbit anti-mouse IgG (Nichirei, Tokyo, Japan), biotinylated goat anti-rabbit IgG (Nichirei), peroxidase-conjugated streptavidin (Nichirei), peroxidaselabeled goat anti-mouse IgG Fab (Nichirei), and peroxidaselabeled goat anti-rabbit IgG Fab (Nichirei) were purchased.

Techniques: Expressing, Cell Culture, Immunofluorescence, Staining, Control

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1) Product Images from "Impaired migration and metastatic spread of human melanoma by a novel small molecule targeting the transmembrane domain of death receptor p75 NTR"

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